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KMID : 0380020150300060296
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Korean Journal of Biotechnology and Bioengineering
2015 Volume.30 No. 6 p.296 ~ p.301
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Production of Recombinant GG1234-DsRed Fusion Protein and Its Effect on in vitro CaCO3 Crystallization
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Son Chae-Yeon
Kim Jin-Ho
Kim Ji-Ha
Choi Yoo-Seong
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Abstract
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Eggshell-based biocomposites have become attractive due to their exquisite nanostructure and biological properties, which are mainly composed of highly organized calcium carbonate crystals controlled by organic macromolecules such as proteins and polysaccharides. Here, we designed the recombinant fusion protein of a putative eggshell matrix protein named as GG1234 and a fluorescent reporter protein of DsRed. The protein was successfully over-expressed in E. coli and purified by Ni-NTA affinity chromatography. In vitro calcium carbonate crystallization was conducted in the presence of the fusion protein, and morphological change was investigated. The protein inhibited the calcite growth in vitro, and spherical calcium carbonate micro-particles with the diameter of about 20-30 μm were obtained. We expect that this study would be helpful for better understanding of eggshellbased biomineralization.
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KEYWORD
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Biomineralization, Calcium carbonate, Recombinant protein, Fusion protein
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